微核(micronucleus,簡(jiǎn)稱MCN)是真核生物細(xì)胞中的一種異常結(jié)構(gòu),是由有絲分裂后期喪失著絲粒的染色體片斷游離于細(xì)胞質(zhì)中產(chǎn)生的。細(xì)胞受遺傳毒物作用形成微核,以檢測(cè)細(xì)胞中微核的形成來(lái)檢測(cè)物質(zhì)遺傳毒性的試驗(yàn)稱為微核試驗(yàn)。目前,微核試驗(yàn)已被許多國(guó)家及機(jī)構(gòu)納入標(biāo)準(zhǔn)檢測(cè)試驗(yàn),用于評(píng)價(jià)新藥、食品添加劑、農(nóng)藥、化妝品、工業(yè)化學(xué)品、環(huán)境污染物等遺傳毒性的檢測(cè)、安全性評(píng)價(jià)和遺傳損害的檢測(cè)。
體外流式微核分析,讓該過(guò)程變得更加簡(jiǎn)單、快捷、可靠,并可獲得更豐富的信息。
流式細(xì)胞術(shù)是檢測(cè)微核的新興技術(shù)平臺(tái)。相比于傳統(tǒng)的顯微鏡下計(jì)數(shù)微核的方法,流式細(xì)胞技術(shù)的檢測(cè)優(yōu)勢(shì)在于:高速高通量,多細(xì)胞多參數(shù),高特異性,多信息。
In Vitro MicroFlow Kits 流式細(xì)胞術(shù)體外微核檢測(cè)試劑
試劑規(guī)格:
IN VITRO-250/50:Analyze 250 samples in 96-well plates or analyze 50 samples in larger format plates or flasks.
IN VITRO-1,000/200:Analyze 1,000 samples in 96-well plates or analyze 200 samples in larger format plates or flasks.
IN VITRO-2,000/400:Analyze 2,000 samples in 96-well plates or analyze 400 samples in larger format plates or flasks.
試劑運(yùn)輸條件:
Ambient
試劑盒存儲(chǔ)條件:
Freezer (-20 °C)
Refrigerator (4 °C)
Ambient
試劑盒組成:
Incomplete Lysis Solution 1: Lyses cells after staining
Incomplete Lysis Solution 2: Lyses cells after staining
Nucleic Acid Dye A: Stains the DNA of cells undergoing apoptosis or necrosis
Nucleic Acid Dye B: Stains nuclei and micronuclei
RNase Solution: Removes RNA so Nucleic Acid Dye B stains only nuclei and micronuclei
10x Buffer: Used in the lysis solutions
相關(guān)材料的要求:
Cell line and cell culture materials
Freezer (-20 °C) and refrigerator (4 °C)
Centrifuge and other general lab supplies
Flow cytometer capable of 488 nm excitation
Light source (fluorescent preferred)
If using 96-well plates, an 8-channel bridge and aspirator manifold (available from V&P Scientific)
Litron Laboratories 相關(guān)產(chǎn)品:
1、Litron Laboratories 流式細(xì)胞術(shù)檢測(cè)試劑 產(chǎn)品目錄
2、In Vitro MicroFlow Kits 流式細(xì)胞術(shù)體外微核檢測(cè)試劑
3、In Vivo MicroFlow Kits 流式細(xì)胞術(shù)體內(nèi)微核檢測(cè)試劑
4、In Vivo MutaFlow Kits 流式細(xì)胞術(shù)大鼠體內(nèi)Pig-a基因突變檢測(cè)試劑
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